Titlebook: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC); Methods and Protocol Bettina Warscheid Book 2014 Springer Science+Business

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Graduated

Global Ubiquitination Analysis by SILAC in Mammalian Cells,uantitative strategies, such as stable isotope labeling with amino acids in cell culture (SILAC). Here we describe in detail a SILAC protocol to profile the ubiquitome in mammalian cells including protein labeling, antibody-based enrichment, and analysis by mass spectrometry.

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,Identifying Nuclear Protein–Protein Interactions Using GFP Affinity Purification and SILAC-Based Qusearch for PPIs. In this chapter, we describe a single-step GFP affinity purification method combined with SILAC-based quantitative mass spectrometry that can be used to identify nuclear PPIs in mammalian cells.

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征稅

Fifteen Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC),dy been developed and used in my laboratory for a number of years. From the beginning, it was applied to challenging problems in cell signaling that were considered out of reach for proteomics at the time. It was also used to pioneer proteomic interactomics, time series and dynamic posttranslational