Titlebook: Single Cell Analysis; Methods and Protocol Miodrag Gu?vi? Book 2024 Springer Science+Business Media, LLC, part of Springer Nature 2024 qPCR

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Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients,heterotypic clusters that contain tumor cells admixed with normal cells are frequently observed in patients with solid tumors. Current methods used for cluster detection and enumeration do not allow an accurate estimation of the relative fractions of tumor cells. Here we describe a method for estima

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Establishing Single-Cell Clones from In Vitro-Cultured Circulating Tumor Cells,metastases. However, CTCs are rare, heterogeneous, and difficult to expand in culture. We have previously created CTC-derived cell lines from stage IV breast cancer patients. These CTC lines were used to establish single-cell CTC clones using flow cytometry cell sorting.

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Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Singlatal diagnosis, as well as for cancer research purpose. For the latter, studies of tumor heterogeneity, circulating tumor cells (CTCs), and disseminated cancer cells (DCCs) require the analysis of single-cell genomes. Here we describe a reliable and robust array-based comparative genomic hybridizati

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Single Cell Micro RNA Sequencing Library Preparation,terization of physiological miRNA functions is an important basic research question, and miRNAs even have high potential as biomarkers both for prognosis and diagnosis. In order to exploit this potential, it is mandatory to accurately quantify the miRNA expression not only in bulk but also on the si

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Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression,e interrelated at the level of single cells has been challenging due to the lack of techniques for multimodal profiling of individual cells. We recently developed Patch-seq, a technique that combines whole-cell patch clamp recording, immunohistochemistry, and single-cell RNA-sequencing (scRNA-seq) t