Titlebook: Single Cell Analysis; Methods and Protocol Miodrag Gu?vi? Book 2024 Springer Science+Business Media, LLC, part of Springer Nature 2024 qPCR

BUMP

Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression,l, and transcriptomic data from single cells. Patch-seq enables researchers to explore the rich, multidimensional phenotypic variability among cells and to directly correlate gene expression with phenotype at the level of single cells.

VAN

Single-Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasislation and recovery of individual viable human metastatic cells from zebrafish embryos xenotransplanted with cancer cells for downstream molecular analysis. We cover the critical steps for the dissociation of the xenografted zebrafish embryos to generate a single-cell suspension, and the micromanipulation for their recovery as single cells.

死亡

antecedence

Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients,ting tumor fraction of clusters including isolation and collection of single clusters, assessment of copy number alterations of single clusters by low-pass whole genome sequencing, and bioinformatic analysis of sequencing data.

改良

GREG

Single Cell Isolation from Surgically Resected Tissue Via Mechanical Dissociation Using TissueGrindsolid tissue are neither robust nor reproducible. Here we describe primary cells isolation from surgically resected tumor tissue via enzyme-free mechanical dissociation using TissueGrinder, a novel semi-automated benchtop device. The isolated cells can be used for any downstream biochemical or cell-

Microaneurysm

,Circulating Tumor Cell Enrichment and Single-Cell Isolation Combining the CellSearch? and DEPArray?ring of treatment response. CTCs can be obtained repeatedly by simple blood draws as so-called “l(fā)iquid biopsy.” Thus, they can serve as a surrogate material for primary or metastatic tissue biopsies. In addition, isolation of CTCs provides the possibility to investigate those cells which may hold th

avarice

羅盤

IRATE

Isolation of Single Circulating Tumor Cells Using VyCAP Puncher System,eity of cancer cells, the use of single-cell isolation technology has had a major boost in recent years, gaining ground to bulk analysis in the study of solid tumors. In the liquid biopsy field, the use of technologies for single-cell analysis has represented a major advance in the study of the hete