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Titlebook: Signal Transduction Protocols; Robert C. Dickson,Michael D. Mendenhall Book 20042nd edition Humana Press 2004

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書目名稱Signal Transduction Protocols
編輯Robert C. Dickson,Michael D. Mendenhall
視頻videohttp://file.papertrans.cn/868/867101/867101.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Signal Transduction Protocols;  Robert C. Dickson,Michael D. Mendenhall Book 20042nd edition Humana Press 2004
描述In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to
出版日期Book 20042nd edition
版次2
doihttps://doi.org/10.1385/1592598161
isbn_softcover978-1-61737-448-7
isbn_ebook978-1-59259-816-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2004
The information of publication is updating

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Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation,quences are associated with the protein of interest in vivo. The ChIP method described here is intended for studying protein-DNA association in the budding yeast ., but it can be easily implemented in other cell types, including fly, mammalian, and plant cells.
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Techniques in Protein Methylation,ine methyltransferases to illustrate different approaches that have been developed to assess protein methylation. Both in vivo and in vitro methylation techniques are described, and the use of small molecule inhibitors of protein methylation will be demonstrated.
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Making Protein Immunoprecipitates, Immunoprecipitation and related methods of affinity purification are routinely used to assess binding partner interactions and enzyme activity in addition to the size of a protein, rates of protein synthesis and turnover, and protein abundance, thus making it a mainstay of a wide variety of protoco
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Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets,tions, providing a good strategy to monitor molecular responses induced by the activation or inactivation of specific signaling pathways. In this chapter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allo
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