Titlebook: Shiga Toxin-Producing E. coli; Methods and Protocol Stephanie Schüller,Martina Bielaszewska Book 2021 Springer Science+Business Media, LLC,

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Isolation and Characterization of Shiga Toxin Bacteriophages,ntify and enumerate Stx phages. Real-time quantitative PCR (qPCR) is the most accurate method for absolute quantification, although it cannot determine the infectivity of Stx phages. qPCR can also be useful for the detection of free Stx phage virions in different sample types..Stx phages induced fro

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Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Recerlay detection allows for sensitive identification of Stx-binding GSLs in complex mixtures directly on the TLC plate. For this purpose, specific anti-GSL antibodies or Stxs themselves in conjunction with anti-Stx antibodies can be used. The described protocols of antibody-mediated detection of TLC-

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Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-Producing , to Epithelial Cening methods used to isolate inhibitory Nbs blocking intimin–Tir protein–protein interaction, actin-pedestal formation, and intimate adhesion of STEC to epithelial cells in vitro. First, we describe how VHH gene repertoires can be produced as Nbs secreted by . using the α-hemolysin (HlyA) protein s

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Lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-Producing , Infectids for the use of CR(Φ.) infection as a highly predictable murine model for infection and disease by STEC. Importantly, we detail CR(Φ.) inoculation by feeding, determination of pathogen colonization, production of phage and toxin, and assessment of intestinal and renal pathology. These methods prov

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Stefan Bletz,Alexander Mellmann,Barbara Middendorf-Baucharty has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst978-3-642-26872-4978-3-642-19382-8Series ISSN 2192-3671 Series E-ISSN 2192-368X

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Kenneth G. Campellone,Alyssa M. Coultery has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst978-3-642-26872-4978-3-642-19382-8Series ISSN 2192-3671 Series E-ISSN 2192-368X