Titlebook: Seed Dormancy; Methods and Protocol Naoto Kawakami,Kazuhiro Sato Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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Assessment of Dormancy Level and Germination Ability of Seeds with Physiological Dormancymination ability of a given set of seeds is prerequisite for most of the studies. However, germination is very sensitive to imbibition conditions, and dormancy development is also sensitive to growth conditions of the mother plants. In this chapter, we describe tips for plant growth and germination

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Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryorfering with the generation of transgenic plants and thus hampering the analysis of gene function. Transient expression in target cells is a useful approach for studying the function of seed dormancy genes. Here, we describe a protocol for transiently expressing genes related to seed dormancy in the

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Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheatany years to produce knockout mutants using conventional techniques in polyploid plants such as hexaploid wheat. Genome editing with sequence-specific nucleases is a promising approach for obtaining knockout mutations in all targeted homoeologs of wheat simultaneously. Here, we describe a procedure