Titlebook: Schwann Cells; Methods and Protocol Paula V. Monje,Haesun A. Kim Book 2018 Springer Science+Business Media, LLC 2018 Cell culture.Genetic m

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Book 2018to four parts. Part I contains protocols for .in vitro .culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create?cancer cell lines and engineered Schwann cells from unconventional sources via chemi

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CODA

Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Sssociated schwannomas. We describe the generation of human Schwann cells (HSCs) with depletion of merlin and their application in high-throughput screening of chemical libraries to identify compounds that decrease their viability. This NF2-HSC model is amenable for use in independent labs and high-throughput screening (HTS) facilities.

tenuous

Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelinatios and DRG neurons, primary cells that are otherwise resilient to direct transfection. Here we present our protocol for lentiviral production and purification and subsequent infection of large numbers of Schwann cells and/or DRG neurons for the molecular study of peripheral nerve myelination in vitro.

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Schlemms-Canal

The Pseudopod System for Axon-Glia Interactions: Stimulation and Isolation of Schwann Cell Protrusios method is compatible with a large array of current biochemical analyses and provides an effective approach to study biomolecules that are differentially localized in Schwann cell protrusions and cell bodies in response to axonal signals. A similar approach can be extended to different kinds of cell-cell interactions.

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