Titlebook: Schizosaccharomyces pombe; Methods and Protocol Teresa L. Singleton Book 2018 Springer Science+Business Media, LLC, part of Springer Nature

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Random Spore Analysis in Fission Yeast,ften the method of choice for constructing strains with unambiguous genotypes. It is also useful to identify the frequency of rare events. Strains are crossed on a nitrogen-limiting medium for three days. Mated cells are observed under the microscope to check for the presence of ripe asci. To releas

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Duplication and Transformation of the , Collection of Deletion Strains,lable collection of . with single-gene deletions. We also describe how to prepare a duplicate collection of the deletion strains in order to preserve the longevity of the master set. These protocols are adapted to the 96-well format of the 3004 strains of the Version 2.0 Bioneer set but can also be

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Schizosaccharomyces pombe Biotechnological Applications in Winemaking,his alcoholic fermentation lactic bacteria . is used to stabilize wine from a microbiological point of view by converting malic acid into lactic acid that it is not a microbiological substract. The yeast species . was traditionally considered spoilage yeast. Nevertheless, during the last decade it s

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Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast, can be used to test the effect of gain-of-function or loss-of-function of a gene product. Here, we describe a streamlined and large-scale molecular cloning strategy for genome-wide characterization of small viruses in fission yeast.