Titlebook: Salamanders in Regeneration Research; Methods and Protocol Anoop Kumar,András Simon Book 2015 Springer Science+Business Media New York 2015

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Husbandry of Spanish Ribbed Newts (,)pter, we present detailed husbandry protocols for the Spanish ribbed newt (.?). We describe the main phases of their life cycle, with emphasis on the progressive development of sensory, motor, and integration systems, which lead to the acquisition of specific stereotyped (and conditioned) behaviors.

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Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studiesconducted in species belonging to the Plethodontidae family, where some of the species differs from other salamander families due to their direct development, thus absence of a larval life. Here, we describe a protocol to maintain the plethodontid salamanders of genus . species under laboratory cond

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Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISHifferentiate and redifferentiate into lens cells. Recent studies have shown that nuclear architecture of PECs is dynamically changed and unique epigenetic regulation in somatic nucleus is crucial in the lens transdifferentiation. Immuno-FISH, detection of protein and gene loci in nucleus, is one of

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Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablations. Neuronal regeneration occurs in a subset of species, including lizards, teleost fish, axolotls, and newts. One tool for studying neuronal regeneration in the adult brain is intraventricular injection of selective neuronal toxins, which leads to loss of subpopulations of neurons. To trace cells in

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The Accessory Limb Model: An Alternative Experimental System of Limb Regenerationy pathways and test for signaling molecules that regulate limb regeneration. Here, we summarize the history of the ALM and describe the specific details involved in inducing ectopic blastemas and limbs from a skin wound on the side of the arm.

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High-Efficiency Electroporation of the Spinal Cord in Larval Axolotlil. Particularly fascinating is their ability to regenerate a fully functional spinal cord after losing the tail. Electroporation of DNA plasmids or morpholinos is a valuable tool to gain mechanistic insight into the cellular and molecular basis of regeneration. It provides among other advantages a

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