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Titlebook: SMC Complexes; Methods and Protocol Anjana Badrinarayanan Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Chro

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Preparation of Cell Cycle-Synchronized , Cells for Hi-Cealed previously unsuspected levels of chromosome structure, with different types of chromosome structure facilitating function at different stages of the cell cycle. Elucidating how SMC complexes regulate these distinct types of organization is currently a key question in molecular biology..The ran
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In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNAdate the molecular mechanisms underlying these roles, it is crucial to determine how and where condensins are loaded to chromosomal DNA. Here, we describe in vivo and in vitro assays for monitoring the topological loading of two bacterial condensins, Smc-ScpAB and MukBEF. A key step in these assays
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A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexesn genome function and structure such as topoisomerases, DNA and RNA polymerases, and DNA repair enzymes contain nucleotide binding domains (NBD) which bind and eventually cleave ATP. The released energy is harnessed in various ways by these enzymes in order to fulfill their essential functions. Howe
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