Titlebook: SILAC; Methods and Protocol Jose L. Luque-Garcia Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Alistair Langlands,Hamze Beati,H.- Arno J. Müllerrganized from both, insect and plant viewpoints. Results are analyzed and illustrated by graphs and diagrams. The main impact of the monograph is seen in entomology, phytopathology, forestry, agriculture ecology and biocontrol.978-3-319-36189-5978-3-319-08410-7

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Aline R. D?rrbaum,Erin M. Schuman,Julian D. Langer

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1064-3745 expertsThis detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of cho

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Book 2023 necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .SILAC: Methods and Protocols. serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science..

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Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quanalysis and interpretation of MS data to identify and select the specific partners interacting with the protein of interest. As an example, this workflow is applied to the discovery of potential interaction partners of glyceraldehyde-3-phosphate dehydrogenase in the gram-negative bacterium ..

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Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC,ome in SCM. In this protocol, the secretome of SFM is compared with that of SCM to confirm the effect of FBS. Additionally, for mass spectrometric data processing, we provide parameters that increase true positives and decrease both false positives and false negatives.

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pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles,pSILAC-based approaches can be used to pulse/trace the origins of EV protein content and thereby provide valuable insight into vesicle biology and likely effects on intercellular communication in diverse settings.

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Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultureled. When combined with bioorthogonal noncanonical amino acid tagging (BONCAT), it allows for comparative proteomic analysis of de novo protein synthesis. Here we describe protocols that utilize the multiplex SILAC labeling strategy for primary cultured neurons to study steady-state and nascent proteomes.