Titlebook: Ribozymes and siRNA protocols; Mouldy Sioud Book 2004Latest edition Humana Press 2004

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Analysis of Global Conformational Transitions in Ribozymes,zymes contain one or more helical junctions, that act as key architectural elements in the structures. A combination of comparative gel electrophoresis and fluorescence resonance energy transfer (FRET) can be used to analyze the global conformation in solution, and to study the ion-induced folding p

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Selection In Vitro of Allosteric Ribozymes,these distinct functional classes may be combined to generate multipartite ribozymes in which the catalytic and ligand-binding elements act coordinately. In this chapter, we describe a procedure known as allosteric selection, in which an RNA catalyst in conjunction with a random sequence domain is s

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Ribozyme Expression Systems,h level of production of ribozymes in vivo. The engineered tRNA.-driven ribozymes, based on computer-predicted secondary structure, were relatively stable and were transported to the cytoplasm, where they could be colocalized with their target RNA. The activity of the exported ribozymes was signific

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