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Titlebook: Riboswitches; Methods and Protocol Alexander Serganov Book 2009 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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發(fā)表于 2025-3-21 16:17:40 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Riboswitches
副標題Methods and Protocol
編輯Alexander Serganov
視頻videohttp://file.papertrans.cn/831/830178/830178.mp4
概述A comprehensive and timely reference volume for basic and applied research on regulatory RNAs.Covers a wide range of assay formats and systems from the computerized prediction of regulatory RNA elemen
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Riboswitches; Methods and Protocol Alexander Serganov Book 2009 The Editor(s) (if applicable) and The Author(s), under exclusive license to
描述The transfer of hereditary information from genes to proteins is one of the essential pr- esses in all living organisms on our planet. Some genes are expressed without modu- tion throughout the life of a cell, while many others require various degrees of control to precisely balance cellular metabolism with environmental conditions. For many years, researchers attributed this regulatory function to protein molecules, which can direct gene expression at multiple levels, in response to various input signals, and with different degrees of selectivity. Even when the control of gene expression was achieved via direct interactions between proteins and mRNAs, the active role was routinely assigned to p- teins, while RNAs were considered merely as recipient molecules. The discovery of RNA interference and multiple bacterial regulatory RNAs caused a shift from the perception of proteins as the predominant regulators of gene expression to the acknowledgement of the importance of RNAs in many regulatory circuits. Such a viewpoint received strong support several years ago after the discovery of riboswitches and related RNA sensors – mRNA regions capable of alternating their conformations in re
出版日期Book 2009
關(guān)鍵詞Ligation; Termination; Translation; enzymes; evolution; gene expression; genes; molecular biology; tRNA
版次1
doihttps://doi.org/10.1007/978-1-59745-558-9
isbn_softcover978-1-61737-947-5
isbn_ebook978-1-59745-558-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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發(fā)表于 2025-3-21 22:59:37 | 只看該作者
Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemica modular design enables fast access to a large number of purine riboswitch derivatives with the modification of interest at any strand position. We exemplarily provide a detailed protocol for the preparation of adenosine deaminase (.) A-riboswitch variants with 2-aminopurine (AP) modifications at the 40-nmol scale.
板凳
發(fā)表于 2025-3-22 02:45:45 | 只看該作者
Transcriptional Approaches to Riboswitch Studies,in biosynthesis and sulfur metabolism. The system can be utilized to facilitate biochemical studies of riboswitches in general, i.e., to simplify analysis of riboswitches that are not necessarily involved in transcriptional control.
地板
發(fā)表于 2025-3-22 07:51:06 | 只看該作者
,Monitoring RNA–Ligand Interactions Using Isothermal Titration Calorimetry,etail this method using the titration of an adenine-binding RNA with 2,6-diaminopurine (DAP) as a practical example. Our insights should be generally applicable to observing the interactions of a broad range of molecules with structured RNAs.
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Predicting Riboswitch Regulation on a Genomic Scale, used to predict new examples of these structured RNAs. Since riboswitches generally function as .-regulatory elements, examining the genomic contexts of these hits is critical for evaluating their biological relevance. With these two sources of comparative support, it is possible to identify ribosw
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發(fā)表于 2025-3-23 07:46:06 | 只看該作者
Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemicact at the transcriptional or translational level and off- or on-regulate genes upon binding to their dedicated ligands. Biophysical studies on ligand-induced folding of these RNA domains in vitro contribute to understanding their regulation mechanisms in vivo. For such studies, in particular, for ap
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