| 書(shū)目名稱 | Rhodopsin | | 副標(biāo)題 | Methods and Protocol | | 編輯 | Beata Jastrzebska | | 視頻video | http://file.papertrans.cn/831/830144/830144.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 叢書(shū)名稱 | Methods in Molecular Biology | | 圖書(shū)封面 |  | | 描述 | .This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation. The first chapters of the book focus on methods developed to study fundamentals of rhodopsin structure and function, starting with improved purification protocols of native and mutated rhodopsin, followed by methods used for rhodopsin reconstitution into lipid bilayers stabilizing rhodopsin function properties, and finally describing recently developed methods to study structural dynamics of rhodopsin activation and its mechanistic properties. Subsequently, chapters underline various techniques that have been developed to visualize the rhodopsin dimer and to study its functional significance. ?The next few chapters highlight cutting-edge imaging techniques of photoreceptors, rhodopsin trafficking, and its diffusion within signaling membranes. Finally the book concludes with recent developments that could be potentially beneficial in patient treatments, and treatment strategies for retinal degenerative diseases. Written in the highly successful .Methods in | | 出版日期 | Book 2015 | | 關(guān)鍵詞 | G-Protein Coupled Receptor; age-related macular degeneration; photoreceptor degenerative diseases; prot | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-2330-4 | | isbn_softcover | 978-1-4939-4936-6 | | isbn_ebook | 978-1-4939-2330-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2015 |
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