Titlebook: Rhodopsin; Methods and Protocol Valentin Gordeliy Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr

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Rhodopsin-Based Optogenetics: Basics and Applications,diseases. First clinical trials for the optogenetic recovery of vision are underway..This chapter provides a comprehensive description of the structure and function of the different light-gated channels and some new light-activated ion pumps. Some of them already play an essential role in optogeneti

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Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications,on of genes across kingdoms with a goal of facilitating the production of the next generation of molecular tools for neuroscience. We then provide a detailed protocol that allows expression and testing of novel genetically encoded tools in mammalian cell lines and primary cultured neurons.

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In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins, this chapter, we describe procedures how to extract membranes from oocytes after cRNA microinjection and heterologous protein expression. Enzymatic activity of these membranes is then analyzed under different illumination conditions. In addition, fluorescent labeling of the rhodopsins is employed t

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1064-3745 ge and thorough, .Rhodopsin: Methods and Protocols. is a valuable resource for any scientist and researcher interested in learning more about this developing field..978-1-0716-2331-2978-1-0716-2329-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Kirill Kovalev,Roman Astashkin,Valentin Gordeliy,Vadim Cherezov