Titlebook: Reverse Genetics of RNA Viruses; Methods and Protocol Daniel R. Perez Book 2017 Springer Science+Business Media LLC 2017 reverse genetics.R

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Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus, genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA-dependant RNA polymerase.

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Plasmid-Based Reverse Genetics of Influenza A Virus, of alternative vaccines and vaccination strategies. In this chapter we describe the cloning of cDNA copies of viral RNA segments derived from a type A influenza virus into reverse genetics plasmid vectors and the experimental procedures for the successful generation of recombinant influenza A viruses.

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Reverse Genetics for Mammalian Orthoreovirus,ines and virus-based vectors. Here, we describe the reverse genetics system that allows for production and recovery of mammalian orthoreovirus, a double-stranded (ds) RNA virus, from plasmids that encode the viral genome.

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BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV),sk populations. Current efforts to develop vaccines include development of live-attenuated RSV candidates. We describe here methods for preparation and recovery of recombinant RSV using an efficient bacterial artificial chromosome (BAC)-based system, expansion and plaque purification of recovered virus, and generation of master and working stocks.

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Book 2017des readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necess

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1064-3745 ation advice from the experts.Includes supplementary materia.This volume is a compilation of sixteen chapters that detail reverse genetics protocols. .Reverse Genetics of RNA Viruses: Methods and Protocols. guides readers through comprehensive protocols on RNA viruses, that were the most challenging

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