Titlebook: Retinoids; Methods and Protocol Hui Sun,Gabriel H. Travis Book 2010 Springer Science+Business Media, LLC 2010 Transport.Vitamin.Vivo.biolog

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The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotroetinoid–RBP complexes, as well as of unliganded RBP, with TTR can be investigated by means of fluorescence anisotropy. RBP represents the prototypic lipocalin, in the internal cavity of which the retinol molecule is accommodated. Due to the tight binding of retinol within a substantially apolar bind

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Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle,n that undergoes activation by light in vertebrate eyes. Pathogenic mutations within genes encoding proteins involved in the retinoid cycle lead to abnormalities in retinoid homeostasis and numerous congenital blinding diseases of humans. Thus, elucidation of disease-specific changes in enzymatic ac

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Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy,ophore, 11-.-retinal, a prosthetic group of rhodopsin that undergoes activation by light. Many different mutations in genes encoding retinoid cycle proteins can cause a variety of human blinding diseases. Two-photon microscopy is an evolving, non-invasive, and repetitive imaging technology that can

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Detection of Retinoic Acid Catabolism with Reporter Systems and by In Situ Hybridization for CYP26 action of all genes and proteins. It functions via two modes: (1) as controlling ligand for specific transcription factors in the nucleus it stimulates or inhibits gene expression from RA response elements in gene promoters; (2) in non-genomic pathways it activates kinase-signaling cascades that con

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Diet in Vitamin A Research,o perspectives: human research, in which diet is usually variable and analysis of dietary intake is paramount to interpreting the study’s results, and animal (rodent) research, in which diet is imposed as a factor in the experimental design, and the diet consumed is usually monotonous. Many standard

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Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigmen degeneration. In the development of approaches to the amelioration of retinal disorders characterized by enhanced RPE lipofuscin formation, attention is being given to therapies that reduce the production of these damaging pigments. An understanding of the biosynthetic pathways by which these molec

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Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina, a G protein-coupled receptor and the photo-sensitive ligand, 11-.-retinaldehyde (11-.RAL). Absorption of a photon by an opsin pigment induces isomerization of the 11-.RAL chromophore to all-.retinaldehyde (all-.RAL), rendering the pigment insensitive to light. The bleached opsin regains light sensi

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1064-3745 ng tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search978-1-61779-690-6978-1-60327-325-1Series ISSN 1064-3745 Series E-ISSN 1940-6029