Titlebook: Restriction Endonucleases; Alfred M. Pingoud Book 2004 Springer-Verlag Berlin Heidelberg 2004 ATP.DNA.DNA-Erkennung.DNA-Spaltung.Restrikti

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The Type I and III Restriction Endonucleases: Structural Elements in Molecular Motors that Process ase, ATPase and DNA translocase. It has been demonstrated that ATP-dependent protein motion along DNA is necessary for endonuclease activity. Studies have shown that Type I enzymes remain bound to their recognition sites whilst simultaneously translocating adjacent non-specific dsDNA past a stationa

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The Integration of Recognition and Cleavage: X-Ray Structures of Pre-Transition State Complex, Postis of DNA(at 24 ° C and pH 7.4) was estimated at 5.7×10. S. (.; .; .); more recently, Radzicka and Wolfenden have estimated this value as 1.7xl0. S.. (.).This corresponds to an estimated half-life of 130,000 years for a DNA phosphodiester bond in solution, placing DNAhydrolysis among the slowest of

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Structure and Function of EcoRV Endonuclease,nt-ended fashion. Like most of the restriction enzymes used as tools in recombinant DNA technology it can be considered as a simple hydrolytic enzyme of relatively small size with precisely defined substrate specificity and the rather common requirement for Mg. ions. Yet, these apparently simple pre

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Two of A Kind: BamHI and BglII,uding EcoRI (.; .), EcoRV (.; .; .; .; .), BamHI (., .; ., .) PvuII (.; .; .) FokI (., .), Cfrl0I (.), BglI (.), BglII (., .), BsoBI (.), NaeI (., .), NgoMIV (.), Bse634I (an isoschizomer of Cfr10I), (.), MunI (.), and HincII (.). All except Cfrl0I and Bse634I have been found to be bound to their co

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Structure and Function of the Tetrameric Restriction Enzymes,, within or close to their recognition sites (.). Around 3500 species, from variety of bacteria with nearly 240 differing specificities, have now been characterized (.). Most of the sequences recognized by Type II restriction endonucleases are palindromic, i. e., possess a twofold rotational axis of

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,Structure and Function of Type IIE Restriction Endonucleases — or: From a Plasmid That Restricts Phn archaea, they often form biologically active DNA restriction-modification (RM) systems (for a review see .).The first R-M systems, discovered by reversible growth reduction of bacterial viruses, were found to be encoded by chromosomal genes in . K-12, ., and . (for reviews: .; .; .). Besides the p

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