Titlebook: Resin Microscopy and On-Section Immunocytochemistry; Geoffrey R. Newman,Jan A. Hobot Book 19931st edition Springer-Verlag Berlin Heidelber

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, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.978-3-642-97481-6

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Book 19931st editionive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section sing

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The Resinsuert et al, 1956; Glauert and Glauert, 1958; Luft, 1961; Glauert, 1965, 1975). The epoxy resins gave uniform curing, and were found to be beam-stable. Uncrosslinked methacrylate is only two-dimensionally bonded and is rapidly destroyed in the electron beam. It is also prone to shrinkage, sometimes u

Tailor

Cryotechniques sensitive to even low concentrations of fixative, or for preserving structures that cannot be stabilised during fixation or dehydration (Sect. 1.3.2). The prerequisite for cryotechniques is for biological tissue to be rapidly frozen prior to any processing steps. Processing can then be in to resin

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Methods for Resin Polymerisation Epoxides can only be cured by heat to produce sectionable blocks. It is possible but impracticable to cure Epoxy resins with UV-light or chemical catalysts (Glauert, 1975). However, considerably longer times are needed for the tissue to be present in an organic monomer (Sect. 1.3.1). These methods

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Strategies in Immunolabelling tissue substances with fluorescent antibodies. Since then numerous ingenious markers have been attached to antisera and other macromolecules in order to identify tissue and cellular substances both in the light and the electron microscope. Ferritin (Singer, 1959) was the first EM marker, followed b

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General Considerationsng, collectively called the section pretreatment, and the staining procedures themselves. The requirement for section pretreatment depends on the extent of fixation and the type of resin, with epoxy resin sections of post-osmicated tissue needing the most attention, and acrylic resin sections of ald

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