Titlebook: Research Methods in Neurochemistry; Volume 2 Neville Marks,Richard Rodnight Book 1974 Springer Science+Business Media New York 1974 attenti

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Isolation and Study of Synaptic Vesiclesnapses. It was proposed, originally by Del Castillo and Katz (1955), de Robertis and Bennett (1955), and Palay (1956), that the vesicles store the quanta of transmitter prior to their release. They are, on this view, the morphological counterparts of the quanta. It has also been proposed that the ve

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Culture of Neural Tissue and Cellsaphy on this subject, I shall not attempt to provide a comprehensive discussion of either the methods employed for such cultures or the information available in the literature on their biochemical properties. I shall, instead, describe the major experimental approaches used and cite representative p

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Measurement of Arteriovenous Differences Across Rat Brain The application of this technique to rats provides data which complement those obtained using the rat brain blower (see Chapter 7), thus providing a more complete metabolic description of brain than is possible in other animals. Furthermore, blood concentrations of many metabolites can be manipulat

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Determination of Transport Rates , tes of movement of a substance from one location to another or the rates of the transformation of a substance from one chemical form to another. I shall thus deal with the planning of such experiments, with the calculation procedures involved, and with the interpretation and significance of the resu

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Isolation of Myelin Basic Proteinsergic encephalomyelitis (EAE; Alvord, 1970). EAE was shown to be an autoimmune disease when Rabat . (1947) induced it in monkeys by injection of autologous brain tissue, together with appropriate adjuvants. Since then, many attempts have been made to isolate the active component of the CNS tissue re

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Methods of Isolation and Identification of Neurophysin Proteins bodies and carried by axoplasmic transport to nerve terminals in the posterior pituitary, where they are stored and released. The hormones are found in association with higher molecular weight proteins, the neurophysins (Fig. 1).

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Methods for the Neurochemical Study of Microtubuleshe morphology or chemistry of these entities in brain. The unusual aspect of their occurrence in nervous tissue is their high concentration. Microtubules are seen in great numbers on electron microscopic examination of neurons. Tubulin concentrations in brain have been reported between 11% and over

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