Titlebook: Regulatory T-Cells; Methods and Protocol Masahiro Ono Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Book 2023ders through,?basic protocols for analyzing?Treg in mice and humans, single-cell analysis for analyzing?Treg, and methods for the analysis of Treg in clinical application.?Written in the format of the highly successful?.Methods in Molecular Biology?.series, each chapter includes an introduction to t

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1064-3745 ation advice from the experts.This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through,?basic protocols for analyzing?Treg in mice and humans, single-cell analysis for analyzing?Treg, and methods

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Adoptive Transfer and Bone Marrow Chimera Models to Analyze Treg Function and Differentiation. Treg function can be quantitatively measured by analyzing the expansion of conventional CD4 T cells and their differentiation into helper cells. The quantitative measure of Treg differentiation is addressed by analyzing the number and phenotype of Foxp3-expressing cells. The use of congenic markers is instrumental for these approaches.

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