Titlebook: Regulatory T-Cells; Methods and Protocol Masahiro Ono Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Analysis of the In Vivo Function of Follicular Regulatory T (TFR) Cells in the Regulation of Antibodsponse and humoral self-tolerance. Although it is generally accepted that T. cells suppress GC antibody responses mediated by follicular helper T (T.) cells and B cells, the helper activity of T. cells on GC responses has also been recently reported. Because of this, it is critical to develop specif

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Adoptive Transfer and Bone Marrow Chimera Models to Analyze Treg Function and Differentiatione transfer and bone marrow chimera to address the effect of a specific mutation on T regulatory cell (Treg) function and differentiation, respectively. Treg function can be quantitatively measured by analyzing the expansion of conventional CD4 T cells and their differentiation into helper cells. The

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Depletion of Treg by the Diphtheria Toxin System describe the methods of depleting regulatory T cells (Tregs) systemically or selectively in the skin. Since it has been difficult to conclude the importance of tissue-residing Tregs with?systemic Treg ablation, we sought to selectively deplete cutaneous Tregs to investigate their function in the sk

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Differentiation of Peripheral Tregells can differentiate into pTreg upon adoptive transfer into Foxp3-diphtheria toxin receptor transgenic recipient mice in which endogenous Tregs are transiently depleted by administration of diphtheria toxin before adoptive transfer. Differentiation of monoclonal pTreg is induced through oral deliv

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