Titlebook: Regulatory B Cells; Methods and Protocol Gaetano Vitale,Francesca Mion Book 2014 Springer Science+Business Media New York 2014 Anti-inflamm

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Regulatory B Cells in Experimental Mouse Models of Arthritisantigen-induced arthritis (AIA) and collagen-induced arthritis (CIA). We will specifically focus on how to induce arthritis as well as methods for the isolation and functional study of Bregs both in vitro and in vivo.

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Regulatory B Cells in Allergic Airways Disease and Asthmare just beginning to be elucidated. Here, we provide detailed instructions for the identification, isolation, and functional characterization of Breg cells in a murine model of allergic airway disease.

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Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functionse in vitro Th1 and Th17 CD4. T cell activation. Here, we provide the methods to analyze and purify the CD24.CD38. transitional B cell subset for further in vitro experiments. We also provide a reliable method to detect B cell IL-10 production using intracellular cytokine staining.

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Use of Cocultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functionstocols to investigate IL-10 production at a single cell level in a dendritic cell-B cell coculture in vitro system. These methods are a proof of concept that can be easily extrapolated and adapted to the study of the interaction between B cells and other immune cell types.

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Fluxes in Forest Ecosystems: Effects of Time, Stressors, and Humans.The volume concludes with a final chapter that reflects on the current state of knowledge and identifies some areas in need of further research..978-94-017-7770-4978-94-007-1363-5Series ISSN 0070-8356 Series E-ISSN 2196-971X