Titlebook: Recombinant Proteins in Plants; Methods and Protocol Stefan Schillberg,Holger Spiegel Book 2022 The Editor(s) (if applicable) and The Autho

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Molecular Farming in Seed Crops: Gene Transfer into Barley (,) and Wheat (,)ging to transform, but considerable progress has been made in enhancing transformation efficiencies and in controlling transgene expression. In this chapter, we describe and discuss standard procedures for generating transgenic barley and wheat for the production of recombinant proteins.

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Cell Biology Methods to Study Recombinant Proteins in Seedsches include sequential extraction procedures, subcellular fractionation and 2D as well as 3D electron microscopy techniques such as electron tomography (ET) and serial block face scanning electron microscopy (SBF-SEM), which are described and discussed in this chapter.

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Production of Recombinant Proteins by ,-Mediated Transient Expressionign and cloning of expression constructs, the transformation, selection, and cultivation of recombinant ., the infiltration of plants using a syringe or vacuum device, and finally the extraction of recombinant proteins from plant tissues.

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Recombinant Protein Production in Plants: A Brief Overview of Strengths and Challenges,rial-scale recombinant protein manufacturing. Later, the production of recombinant proteins was demonstrated in whole plants, which differ morphologically from cell-based systems and require completely different cultivation conditions. Over time, additional plant-based production platforms were esta

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