Titlebook: Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Phy; Selected articles fr O.-W. Merten,D.

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Metabolic Approaches for the Optimisation of Recombinant Fermentation Processesity and the acceleration of process development. The overall strategy for process optimisation is designed to exploit the potential of the host cell by means of optimal control of the flux-ratios between biosynthesis of host cell proteins and recombinant proteins. To optimise the rate of recombinant

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Expression and Fermentation Strategies for Recombinant Protein Production in , valuates new procedures described in the current literature which could be used for the production of recombinant proteins. The known expression systems are discussed with a special focus on alternative strategies which can be applied under process relevant conditions. The second part presents diffe

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Overexpression of a , Lipase in , Strains Containing Multiple Copies of the Target Gene development of a stable . strain containing multiple copies of the heterologous gene, .. This strain was obtained after transforming . with the expression vector pPICZαA-ROL, and subsequent selection of strains containing multiple vector insertion events in the presence of higher Zeocin concentrati

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Protein Mass Production in Hybridomas and Recombinant CHO Cellsction pressure have been investigated. Transcriptional activities and antibody expression of both systems, hybridomas and recombinant CHO cells are compared. Long-term stability of different recombinant glycoprotein expressing CHO cell lines are also shown.

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