Titlebook: Recombinant Protein Production in Yeast; Brigitte Gasser,Diethard Mattanovich Book 2019 Springer Science+Business Media, LLC, part of Spri

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Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Pa other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using . as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally

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Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification,ed. The process consists of a centrifugation step and a depth filtration, followed by a (sterile) membrane filtration. The procedure yields supernatant ready for the next purification step such as chromatography.

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Purification of Recombinant Glycoproteins from , Culture Supernatants,ed efficiently by only a few conventional chromatography steps, the purification of glycosylated recombinant proteins is a very challenging process due to the intrinsic feature of the yeast of hypermannosylation. The resulting vast glycosylation pattern on the recombinant target protein masks its ph

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Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts,ein production. Special focus is given to the metabolic impact of protein production. Furthermore, strategies that were shown to enhance secretion of recombinant proteins in different yeast species are presented.