Titlebook: Recombinant Protein Production in Yeast; Brigitte Gasser,Diethard Mattanovich Book 2019 Springer Science+Business Media, LLC, part of Spri

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Promoters, a constitutive promoter. Recent literature showed that newly identified promoters of . are promising as well, in addition to pAOX1 and pGAP..In this chapter, the regulation mechanisms of inducible pAOX1 and constitutive pGAP promoters are discussed. In addition, here we present an overview about th

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CRISPR/Cas9-Mediated Homology-Directed Genome Editing in , including simultaneous overexpression of multiple genes. To meet this challenge, we have developed a CRISPR/Cas9-based kit for gene insertions, deletions, and replacements, which paves the way for precise genomic modifications in .. In this chapter, the versatile method for performing these modific

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Brain-Imaging

Yeast Surface Display and Cell Sorting of Antigen-Binding Fc Fragments,ding to multiple antigen molecules, require modifications of traditional affinity maturation strategies. Preferred to equilibrium selections are kinetically driven antigen selections, designed to specifically influence the binding off-rate, which in many cases augments the desired biological effect.

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Efficient Development of a Mixed Feed Process for ,,ulation and guarantee highest productivity. Here, we present an efficient strategy comprising only one experiment to determine the settings of such a mixed feed system based on the physiology of the respective yeast strain.

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1064-3745 and Protocols.?aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.?.978-1-4939-9024-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

Dna262

Burcu Gündüz Ergün,Damla Hücceto?ullar?,Sibel ?ztürk,Eda ?elik,P?nar ?al?k

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