Titlebook: Recombinant Protein Expression in Mammalian Cells; Methods and Protocol David L. Hacker Book 2024Latest edition The Editor(s) (if applicabl

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Transient Co-expression of Membrane Protein Complexes in Mammalian Cells,ystem. The protocol can be applied for small-scale screening of any membrane protein alone or co-expressed with interacting partners followed by large-scale production and purification of a potential membrane protein complex.

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Recombinant Protein Production from Stable CHO Cell Pools,media and polyethylenimine (PEI) as the transfection reagent. As a specific example of how this protocol can be applied, the production and downstream purification of recombinant His-tagged trimeric SARS-CoV-2 spike protein ectodomain (SmT1) are described.

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CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells,ecombinant protein productivity. In this chapter, we present a protocol for targeting miRNAs in CHO cells using CRISPR/Cas9 and the analysis of the resulting phenotype, using miR-27 as an example. We show that it is possible to target miRNAs in CHO cells and achieved ≥80% targeting efficiency. Indel

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High-Throughput Protein Expression Screening of Cell-Surface Protein Ectodomains,ioritize well-expressing construct for large-scale production. By employing this method, one can efficiently detect clones with low expression levels, streamlining the process and saving valuable time in identifying optimal candidates for further study.

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ding to the real ones in terms of internal pressure, temperature, corrosion, etc., which has been of decisive importance for the transferability of the test results to real components. In addition, a series of complementary issues has been investigated (material science aspects, nondestructive exami

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Yashas Rajendrading to the real ones in terms of internal pressure, temperature, corrosion, etc., which has been of decisive importance for the transferability of the test results to real components. In addition, a series of complementary issues has been investigated (material science aspects, nondestructive exami

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Julia Flock,Yexin Xie,Régis Lemaitre,Karine Lapouge,Kim Remans Field Service Engineer. The inference engine used a mixed-mode configuration, capable of running in either the forward or backward mode. The Help System provided the operator with assistance by displaying textual information, CAD diagrams or repair sequences from a video disk. The rules were writte