Titlebook: Recombinant Glycoproteins; Methods and Protocol Steven B. Bradfute Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusi

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A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its oiled-coil capture strategy that reduces artifacts. In this method, ACE2 receptors were produced with an E5-tag and immobilized as ligands in the SPR assay. This chapter details methods for high-yield production and purification of the studied proteins, functionalization of the sensor chip, conduction of the SPR assay, and data analysis.

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