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Titlebook: Recombinant Gene Expression; Reviews and Protocol Paulina Balbás,Argelia Lorence Book 2004Latest edition Humana Press 2004

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發(fā)表于 2025-3-21 16:14:29 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Recombinant Gene Expression
副標題Reviews and Protocol
編輯Paulina Balbás,Argelia Lorence
視頻videohttp://file.papertrans.cn/825/824091/824091.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Recombinant Gene Expression; Reviews and Protocol Paulina Balbás,Argelia Lorence Book 2004Latest edition Humana Press 2004
描述Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation,
出版日期Book 2004Latest edition
版次2
doihttps://doi.org/10.1385/1592597742
isbn_softcover978-1-61737-460-9
isbn_ebook978-1-59259-774-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2004
The information of publication is updating

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Back to Basicsand few other bacteria. The early characterization of the molecule, including its nucleotide sequence, replication and maintenance mechanisms, and determination of its coding regions, accounted for its success, not only as a universal cloning vector, but also as a provider of genes and an origin of
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Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriVion level is very low, which is important for the maintenance of any “toxic” genes. This vector could be used in most . hosts. Similar versions of the described vector employ the rhamnose-inducible .. promoter (..). Other expression systems allow independent regulation of the plasmid amplification a
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Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family ,group. In this review, the most relevant tools are described, with emphasis given to cloning vectors, genetic exchange mechanisms, mutagenesis approaches, and reporter genes. Due to its relevance for genetic studies, complementary sections describing the influence of salinity on the susceptibility o
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