Titlebook: Receptor-Receptor Interactions in the Central Nervous System; KJELL FUXE,Dasiel O. Borroto-Escuela Book 2018 Springer Science+Business Med

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純樸

,Analysis and Quantification of GPCR Allosteric Receptor–Receptor Interactions Using Radioligand Bining site. In view of the complex nature of allosteric mechanisms, the detection, analysis, and quantification of the effects of this phenomenon rely on the use of competition radioligand binding assays to ensure proper demonstration of the high and low affinity D2R agonist binding sites. Outlined in

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脫離

,Electrophysiological Approach to GPCR–RTK Interaction Study in Hippocampus of Adult Rats,–RTK interaction in hippocampus CA1 pyramidal neurons of adult rat, paying particular attention to highlight major problems that can occur using this technique and providing useful troubleshooting steps to achieve reliable results.

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Double Fluorescent Knock-In Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Di in which the MOP-DOP heteromers are expressed. Here, we describe a method to quantitatively and automatically analyze changes in the subcellular distribution of MOP-DOP heteromers in primary hippocampal culture from this mouse model. This approach provides a unique tool to address specificities of

極深

Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-ImmunoprDISC1 interactions and discussed their association in the pathophysiology of schizophrenia. This chapter aims to provide systemic guidelines for the standard biochemical techniques in identifying D2R-associated protein–protein interactions, and to investigate the roles of these interactions in the b

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Bimolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerizatiy fusing the fragments to two interacting proteins. The advantage of BiFC over alternative resonance energy transfer techniques is a high signal-to-noise ratio due to its strong intrinsic fluorescence without exogenous fluorogenic or chromogenic agents required. Here we provide a detailed descriptio

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Assessing GPCR Dimerization in Living Cells: Comparison of the NanoBiT Assay with Related Bioluminells. Interestingly, most of the approaches employ noninvasive fluorescence- and luminescence-based assays. Here, we present an efficient strategy to study GPCR dimerization dynamics, namely a protein complementation assay (PCA) based on the reconstitution of a luminescent protein, the NanoLuciferase