Titlebook: Receptor and Ion Channel Detection in the Brain; Rafael Lujan,Francisco Ciruela Book 2021Latest edition The Editor(s) (if applicable) and

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Subsynaptic Membrane Fractionationto access the localization of proteins within synapses, which we exemplify in rodent and human brain tissue. The procedure begins with the fractionation of synaptosomes (synaptic compartments) and then combines light solubilization with mild detergents together with alterations of pH to allow a sepa

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Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cellsembrane. Although classical fluorescence ensemble approaches have provided valuable insights into cellular and molecular pathways, their inherent limitations (e.g., average behaviors, spatial resolution) have prompted neuroscientists to use and adapt other imaging approaches. Among these, the single

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Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brainins. For this purpose, expression vectors based on Semliki Forest virus, Sindbis virus, and Venezuelan equine encephalitis virus, all members of the . genus, have been engineered in the form of naked RNA replicons, recombinant viral particles, and layered DNA vectors. By applying these alphavirus ve