Titlebook: Receptor Signal Transduction Protocols; R. A. J. Challiss Book 1997 Springer Science+Business Media New York 1997

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Stephen R. Ikedaetrien werden zur Qualit?tssicherung u.?a. anhand relevanter Bauteilmerkmale geprüft. Die Lageprüfung misst die Ist-Position eines Merkmals in einer r?umlichen Dimension. Informationen über die Ausrichtung und Geometrie des Brennraums k?nnen durch die eingesetzten Messverfahren nicht abgeleitet werd

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Catheter

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Approaches to the Stable Transfection of G Protein-Coupled Receptorswn, constant quantity of the transfected receptor is expressed for many generations. Both stably transfected pools and clonal cell lines can be studied. Stably transfected pools, or clonal cell lines, that express high levels of a transfected receptor facilitate ligand binding studies; cell lines ex

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Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation ises, and cGMP phosphodiesterase (.). The a-subunit of G protein has inherent high-affinity or low-K. GTPase activity, which hydrolyzes GTP on the a-subunit into GDP and inorganic phosphate (P,) (.). This turn-off reaction results in reassociation of GDP-bound a-subunit and βγ-subunits to end the act

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Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons b in a given cell (e.g., as determined by mnnunohistochemistry) is not sufficient to allow unambiguous assignment of a response to a particular receptor subtype, since additional uncharacterized members of a receptor family may exist. Thus, assigning roles to, and characterizing signaling pathways of

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Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primar, and antibody against Gα. inhibited muscarinic and bradykinin receptor effects on the M-type K. current (.,.). However, this approach is seriously limited by the close sequence homologies between members of G protein a-subunit subfamilies (.), which results in the antibodies being unable to discrim