Titlebook: Reactive Oxygen Species in Plants; Methods and Protocol Amna Mhamdi Book 2022 Springer Science+Business Media, LLC, part of Springer Nature

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Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probecluding formation of abasic sites (AP-sites). Recently, the level of AP-sites in RNA has been measured with the use Aldehyde Reactive Probe (ARP). In the present chapter, we describe application of this technique for the evaluation of the level of AP-sites in plant transcripts.

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Determination of Reactive Carbonyl Species, Which Mediate Reactive Oxygen Species Signals in Plant Ccause distinct kinds of RCS act differently in the cell and so are metabolized, identification and quantification of each RCS in plant tissues provide central information to decipher biochemical mechanisms of plant responses to ROS. This article illustrates practical methods of plant sample preparation and extraction and analysis of RCS.

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Measurement of NAD(P)H and NADPH-Generating Enzymes and homeostasis during episodes of stress. This is especially the case for NADPH, which must be generated from its oxidized form, NADP., by key dehydrogenases. Here, we describe methods that can be used to assay contents and redox states of NAD(H) and NADP(H), as well as simple assays to measure the capacity of two key NADPH-generating enzymes.

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In Vitro Biochemical Analysis of Recombinant Plant Proteins Under Oxidation prepare reduced and oxidized proteins for activity assay, and to characterize the protein under reducing and oxidizing conditions, with a focus on thiol-based oxidative?modifications, like?S-sulfenylation and disulfide formations.

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Book 2022sponses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as

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Analysis of Ascorbate Metabolism in Arabidopsis Under High-Light Stressxidative stress. Thus, ascorbate metabolism plays a key role in photooxidative stress acclimation. Herein, the present authors’ preferred protocols for the application of high-light stress and the measurement of ascorbate and the activity of related enzymes are described.

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