Titlebook: Ras Signaling; Methods and Protocol Lorenza Trabalzini,Saverio Francesco Retta Book 2014 Springer Science+Business Media, LLC 2014 Cell sig

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Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases,ated by Ras GTPases have successfully used the yeast two-hybrid system or related methods to isolate and identify new binding partners of Ras proteins..Here we describe the basic protocol for a yeast two-hybrid library screening and for a small-scale yeast two-hybrid assay.

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A Novel Method for the Production of Fully Modified K-Ras 4B, other, bind nucleotides, and localize in cellular compartments. Given the importance of post-translational modifications in protein biology, development of methods to produce post-translationally modified proteins for biochemical and biophysical studies is timely and significant. At the same time,

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Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation,ances in intensifying screens have allowed for the easy visualization of tritium without the need for extended exposure times. While more energetic radioisotopes are easier to visualize, the lack of commercial source and need for shielding make them more difficult to work with. Since radiolabeled pa