Titlebook: Rapid Cycle Real-Time PCR — Methods and Applications; Genetics and Oncolog Wolfgang Dietmaier,Carl Wittwer,Natarajan Sivasubr Book 2002 Spr

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perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization p

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ted as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!978-3-642-63965-4978-3-642-59397-0

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SYBR Green I Analysis of the Trinucleotide Repeat Responsible for Huntington’s Diseasethe presence of SYBR Green I is used to determine the product T. that is dependent on product length. In addition, we compared melting curves obtained on the ABI 5700 with those obtained on the LightCycler instrument.

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Introduction for Genetics and Oncology Volume Rapid Cycle Real-Time PCR ,f genes includes both altered levels of normal gene products and modified proteins resulting from mutations in exons. In either case, detection of variant expression during early pathogenesis can provide a rational therapeutic approach.

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