Titlebook: Radioimmunoassays for Insulin, C-Peptide and Proinsulin; Lise G. Heding Book 1988 Lise G. Heding 1988 insulin.diabetes

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Radioimmunological Determination of Human C-Peptide in Serumman b-component. Nine out of 12 animals produced useful antibodies within 6 months. Insulin antibodies coupled to Sepharose were used to bind human proinsu-lin and insulin in the serum and after centrifugation C-peptide was determined in the supernatant. The detection limit of the assay (calculated

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Insulin, C-peptide, and Pro insulin in Nondiabetics and Insulin-treated Diabeticsoinsulin (in pmol/ml.) in the 46 non-diabetics were 0.054 ± 0.027, 0.39 ± 0.12, and 0.014 ± 0.009, respectively. The molar ratio between C-peptide and IRI varied from 4.0 to 16.7, with a mean value of 7.9 ± 3.4. Proinsulin constituted 5–36 per cent of IRI, with a mean of 20 ± 10 per cent..Sera from

Extort

The present state and expected future development of methods for the determination of ,-cell secretoy harmless. On the other hand, the long shelf-life of the enzyme reagent, the speed and capacity are a great advantage, and a most promising ELISA for proinsulin was recently described., in which the ever-present non-specific serum interference seems to have been solved.

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