Titlebook: RT-PCR Protocols; Joe O’Connell Book 20021st edition Humana Press 2002

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1064-3745 undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to ra

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Using the Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in was provided by Wang et al. (.). As an internal standard, an RNA template with the same primer sites as the target mRNA was reverse-transcribed. The amplified PCR products differed in size and were separated by agarose gel electrophoresis. The presence of .P-labeled 5′ primer allowed quantification by scintillation counting.

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Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytesding murine macrophages (.,.), human endothelial cells (.,.), eosinophils (.), and Leydig cells derived from human and mouse testis (.). We have demonstrated that human monocytes and lymphocytes express the SP gene mRNA transcripts and produce SP protein (.–.).

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Semi-Quantitative Detection of Hepatitis C Virus RNA by “Real-Time” RT-PCR-α/ribavirin combination therapies, patients with a high viral load respond more poorly to this treatment and require a longer duration of therapy. In addition, the HCV-RNA status in serum during treatment is used as a marker for the short-term success of therapy.

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Nested RT-PCRer amplicon (.). Because their sequences are different from the primary set of primers, they will not amplify artifacts or nonspecific products generated in the primary PCR, enabling product specificity to be maintained over the high number of amplification cycles combined in the primary and secondary PCRs.

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Joe O’ConnellIncludes supplementary material:

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