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Titlebook: ROS Signaling in Plants ; Methods and Protocol Francisco J. Corpas,José M. Palma Book 2024 The Editor(s) (if applicable) and The Author(s),

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發(fā)表于 2025-3-21 18:49:00 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱ROS Signaling in Plants
副標(biāo)題Methods and Protocol
編輯Francisco J. Corpas,José M. Palma
視頻videohttp://file.papertrans.cn/821/820240/820240.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: ROS Signaling in Plants ; Methods and Protocol Francisco J. Corpas,José M. Palma Book 2024 The Editor(s) (if applicable) and The Author(s),
描述This detailed volume presents methodologies for studying reactive oxygen species (ROS) metabolism in plant cells, including techniques for detecting different types of ROS such as superoxide radicals, hydrogen peroxide or singlet oxygen, as well as ROS generating systems such as xanthine oxidoreductase. The book provides alternatives to determine malondialdehyde, electrochemical detection approaches for determining total antioxidant capacity (TAC) as well as enzymatic antioxidants, as well as methods for studying non-enzymatic antioxidants and some components of the secondary metabolism. Written for the highly successful .Methods in Molecular Biology. series, the chapters in this book provide the kind of detailed implementation advice that leads to ideal results in the laboratory.?.Authoritative and practical, .ROS Signaling in Plants: Methods and Protocols. serves as an ideal guide for scientists interested in the metabolism of ROS in plant cells..
出版日期Book 2024
關(guān)鍵詞Reactive oxygen species; ROS metabolism; Antioxidant mechanisms; Oxidative stress; Plant cells
版次1
doihttps://doi.org/10.1007/978-1-0716-3826-2
isbn_softcover978-1-0716-3828-6
isbn_ebook978-1-0716-3826-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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沙發(fā)
發(fā)表于 2025-3-21 21:26:01 | 只看該作者
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發(fā)表于 2025-3-22 02:50:53 | 只看該作者
Francisco J. Corpas,José M. PalmaIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
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發(fā)表于 2025-3-22 06:56:46 | 只看該作者
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Common Methods of Extraction and Determination of Phytomelatonin in Plants,is on the techniques of extraction, cleaning, separation, detection, identification, and quantification. Multiple examples and recommendations are presented for a clear overview of the pros and cons of phytomelatonin determinations in plant tissues, seeds, and fruits, mainly.
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發(fā)表于 2025-3-22 23:37:36 | 只看該作者
Detection of Ascorbate Peroxidase (APX) Activity in Plant Tissues: Using Non-denaturing PAGE and Sping polyacrylamide gel electrophoresis (PAGE) in combination with a spectrophotometric assay for APX activity, the protocol allows identifying diverse APX isozymes present in different organs and plant species.
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發(fā)表于 2025-3-23 02:41:28 | 只看該作者
Determination of ROS Generated by , Xanthine Dehydrogenase1 (AtXDH1) Using Nitroblue Tetrazolium (Nch as nitrogen starvation, wounding, or UV-C. Additionally, we describe the use of NBT staining for detecting enzymatic generation of ROS in native and native SDS PAGE gels. Our protocol also outlines the separation and comparison of the origin of ROS generated by xanthine dehydrogenase1 (XDH1) using different substrates.
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發(fā)表于 2025-3-23 09:02:16 | 只看該作者
Inactivation of Three Stacked Genes of Cytosolic Peroxiredoxins by Genome Editing, in close vicinity on chromosome 1 so that crossing over between the genes most rarely occurs and successful crossing of the plants appears impossible. Genome editing instead allows targeting of multiple isoforms and knocks out several genes at once. This chapter describes how to inactivate the three cytosolic peroxiredoxins by CRISPR/Cas9 in ..
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