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Titlebook: RNAi and Plant Gene Function Analysis; Methods and Protocol Hiroaki Kodama,Atsushi Komamine Book 2011 Springer Science+Business Media, LLC

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書(shū)目名稱(chēng)RNAi and Plant Gene Function Analysis
副標(biāo)題Methods and Protocol
編輯Hiroaki Kodama,Atsushi Komamine
視頻videohttp://file.papertrans.cn/821/820222/820222.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: RNAi and Plant Gene Function Analysis; Methods and Protocol Hiroaki Kodama,Atsushi Komamine Book 2011 Springer Science+Business Media, LLC
描述.The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, .RNAi and Plant Gene Function Analysis: Methods and Protocols. seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful .Methods in Molecular Biology.? series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls..?.In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. .RNAi and Plant Gene Function Analysis: Methods and Protocols. will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing expe
出版日期Book 2011
關(guān)鍵詞Functional Genomics Studies; Gene Silencing; Plant Biology; Plant Engineering; RNA Sequencing; RNA Transf
版次1
doihttps://doi.org/10.1007/978-1-61779-123-9
isbn_softcover978-1-62703-932-1
isbn_ebook978-1-61779-123-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
The information of publication is updating

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Overview of Plant RNAi,tes. Many events that were already known, such as pathogen-derived resistance, posttranscriptional gene silencing, and microRNA (miRNA)-mediated regulation, were found to share a fundamentally similar mechanism. By taking advantage of such mechanisms, whether deliberately or not, we can suppress som
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Heat-Inducible RNAi for Gene Functional Analysis in Plants,a powerful technology for gene functional analysis. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability or plant growth and alternative silencing strategies avoiding these limitations should be preferred. Tissue-specific and induc
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Virus-Induced Gene Silencing in Ornamental Plants,s are engineered to include fragments of target host genes, and the infected plant recognizes and silences the target genes as part of its viral defense mechanism. The consequences of gene inactivation, even of key metabolic, regulatory, or embryo-lethal genes, can thus be readily analyzed. A number
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Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of ,,sient RNAi effects in plants have been achieved via generating stable transformants expressing dsRNAs or artificial microRNAs (amiRNAs) . or by viral-induced gene silencing (VIGS). Although these tools provide outstanding resources for functional genomics, they require generation of vectors expressi
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