Titlebook: RNAi Strategies for Pest Management; Methods and Protocol Luis María Vaschetto Book 2022 The Editor(s) (if applicable) and The Author(s), u

使厭惡

In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control, genes is a crucial initial step. In this chapter, I describe the crucial procedure of in silico screening of the most a5ttractive target genes for RNAi-based pest control, including gene or protein homology comparison, specificity optimization of the dsRNAs to target pests to minimize off-target effect, and primer design.

交響樂

Visualization of RNA Transcripts in Western Corn Rootworm (, and Plants by In Situ Hybridization,ctioning, target recovery, and chromogenic detection of an mRNA (DvSSJ1), encoding for a membrane protein associated with the smooth septate junction (SSJ) in Western corn rootworm [.]. Further, we demonstrate, the expression of dsRNA of DvSSJ1 in maize root tissues using signal amplification and background suppression technology.

保守

Enrage

巨碩

Functional Characterization of Silkworm PIWI Proteins by Embryonic RNAi,A-injected single embryo. The siRNA-injected embryo can be molecularly sexed by W chromosome-specific DNA markers. Using complementary DNA (cDNA) reverse transcribed from the sexed RNA, we evaluated the knockdown effect of the PIWI protein-coding genes on a sexual development-related gene, ..

清晰

步兵

mucous-membrane

Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peaptibility of RNAi-treated populations compared to controls, the target gene may have a direct role in the development of resistance to the insecticide or the gene may be involved in other metabolic processes that may be required for resilience against the insecticide.

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