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Titlebook: RNA‘Protein Interaction Protocols; Susan R. Haynes Book 19991st edition Humana Press 1999

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Measuring Equilibrium and Kinetic Constants Using Gel Retardation Assays,the free (uncomplexed) RNA and protein components of the RNP. This can be formalized by the equilibrium equation: . where R represents the free RNA, P the free protein, and RP the RNA-protein complex for the simplest possible case of a 1∶1 molar complex of RNA and protein. Thus, at equilibrium, the
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PACE Analysis of RNA-Peptide Interactions, DNA and RNA (.). Polyacrylamide coelectrophoresis (PACE) involves electrophoresis of a labeled nucleic acid through a gel medium that contains the target peptide or protein ligand. In this way, the nucleic acid is maintained in a constant concentration of the ligand throughout the electrophoresis,
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An ,-Based Genetic Strategy for Characterizing RNA Binding Proteins, is the increased recognition that RNA-BPs are involved in a wide variety of critical viral and cellular processes including transcription, translation, RNA processing, RNA transport, gene regulation, and splicing. A second reason lies in the technical advances and the generation of new methods that
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