Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2016 Springer Science+Business Media New York 2016 RNA-bindi

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創(chuàng)作

,Biotin–Streptavidin Affinity Purification of RNA–Protein Complexes Assembled In Vitro,ion of RNA in nuclear extract and fractionated using gel filtration, and RNP fractions are pooled for biotin–streptavidin affinity purification. The amount of RNA–protein complexes purified following this protocol is sufficient for mass spectrometry.

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mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA-Binding Protei motif conservation, RNA secondary structure, and the tendency of RBP binding sites to cluster together. A probabilistic model is learned from bona fide binding sites determined by CLIP and applied genome wide to generate high specificity binding site predictions.

破譯密碼

1064-3745 sults.Contains key notes and implementation advice from the .This detailed volume explores the continuing techniques ofstudying RNA-protein complexes and interactions as research in these areasexpand.? After an introductory chapter,the book continues with ways to purify RNA-protein complexes assembl

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,Characterization of RNA–Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF the SR protein family were once thought to have redundant functions, in-depth biochemical analysis of their RNA-binding abilities has revealed distinct binding profiles for each SR protein, that often lead to either synergistic or antagonistic functions. SR protein family members SRSF1 and SRSF2 ar

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Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification),RBPs and ncRNAs jointly influence all aspects of posttranscriptional metabolism, including pre-mRNA splicing and maturation, mRNA transport, editing, stability, and translation. Given the impact of mRNA-interacting molecules on gene expression, there is great interest in identifying mRNA-binding fac

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