Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2016 Springer Science+Business Media New York 2016 RNA-bindi

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Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichmentidentifying yeast mRNA-binding proteins in a systematic manner using UV crosslinking, purification of polyA(+) mRNAs under denaturing conditions, and mass spectrometry to identify covalently bound proteins.

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,Purification of RNA–Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reactihere to prepare splicing-active extracts from whole cells is particularly useful when studying pre-mRNA splicing in various cell types, and the expression of a tagged spliceosomal protein allows one to purify and analyze the RNA–protein complexes by simple immunoprecipitation.

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Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Spling factors with diverse activities in regulating different types of alternative splicing. The artificial splicing factors can be used to change splicing of either minigenes or endogenous genes in cultured human cells, providing a new strategy to study the regulation of alternative splicing and function of alternatively spliced products.

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Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles,ny target site. Here, we describe a method for the reconstitution of functional box H/ACA RNPs using designer box H/ACA guide RNAs, which in principle can be adopted to reconstitute other RNA–protein complexes as well.

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