Titlebook: RNA Tagging; Methods and Protocol Manfred Heinlein Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 RNA-binding

小臼

Ballad

細(xì)頸瓶

Myosin

阻止

LOPE

RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi fused to a fluorescent protein. Several such reporter systems have been described based on RBPs that recognize RNA stem-loops. Here we describe RNA tagging for imaging with an inactive mutant of the bacterial endonuclease Csy4, which has a significantly higher affinity for its cognate stem-loop tha

nerve-sparing

Utilizing Potato Virus X to Monitor RNA Movementthe whole plant via the phloem cell system. Monitoring the movement of these RNA signals can be difficult and time consuming. Here we describe a simple, virus-based system for surveying RNA movement by replacing specific sequences within the viral RNA genome of . (PVX) that are critical for movement

食料

噴出

Synthesizing Fluorescently Labeled dsRNAs and sRNAs to Visualize Fungal RNA Uptaker, most current disease management approaches rely on chemical fungicides that can leave dangerous residues in the environment. RNA interference (RNAi) is an important process through which RNA molecules target and silence complementary genes, regulating gene expression during both transcription and

Chemotherapy

Labeling of dsRNA for Fungal Uptake Detection Analysisest control. A simple and effective method to monitor dsRNA uptake by fungi is crucial for the use of dsRNA as alternative fungicide. The protocol reported in this chapter describes an efficient method to detect and localize labeled dsRNA in fungal hyphae. We use the fungal ., a fungal plant pathoge