Titlebook: RNA Scaffolds; Methods and Protocol Luc Ponchon Book 2015 Springer Science+Business Media New York 2015 DSL ribozyme.RNA origami.RNA protei

BAIL

污穢

大罵

In Vivo Production of Small Recombinant RNAs Embedded in a 5S rRNA-Derived Protective Scaffold,n vitro. Herein, we describe an alternative approach in which RNAs of interest are expressed as a fusion with a 5S rRNA-derived scaffold. The scaffold provides protection against cellular ribonucleases resulting in cellular accumulations comparable to those of regular ribosomal RNAs. After isolation

行業(yè)

,Detection of RNA–Protein Interactions Using Tethered RNA Affinity Capture,ssing. To investigate the functions of these transcripts highly efficient methods are needed to analyze their interactions with RNA-binding proteins (RNBPs), and to understand the binding mechanisms. Many methods have been described to identify RNBPs, but none are wholly satisfactory, in part becaus

Galactogogue

LUDE

Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensoruorescent biosensors are based on receptor proteins fused to fluorescent protein domains. More recently, small molecule-binding riboswitches have been adapted for use as fluorescent biosensors through fusion to the in vitro selected Spinach aptamer, which binds a pro-fluorescent, cell-permeable smal

jocular

嬉耍

,Artificial Ligase Ribozymes Isolated by a “Design and Selection” Strategy,ucture (scaffold) is initially designed, and then a relatively short randomized sequence is installed at the reaction point of the scaffold, followed by the in vitro selection. This method can reduce the length of randomized sequence, providing large coverage of the sequence space in contrast with t

奇怪

Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vi aptamer domain and a self-cleaving ribozyme. The utilization of aptazymes for conditional gene expression displays several advantages over employing conventional transcription factor-based techniques as aptazymes require minimal genomic space, fulfill their function without the need of protein cofa

osteopath

Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells,itch libraries are constructed by randomizing the key nucleotides that couple the molecular recognition function of an aptamer with the self-cleavage activity of a ribozyme. The allosteric ribozyme (aptazyme) candidates are cloned in the 3′ untranslated region (UTR) of a reporter gene mRNA. The plas