Titlebook: RNA Scaffolds; Methods and Protocol Luc Ponchon Book 2015 Springer Science+Business Media New York 2015 DSL ribozyme.RNA origami.RNA protei

NAUT

1064-3745 ethods and Protocols .will serve as an invaluable reference for those interested in further study into this fascinating field..978-1-4939-5589-3978-1-4939-2730-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Margueritte El Khouri,Marjorie Catala,Bili Seijo,Johana Chabal,Carine Tisné,Frédéric Dardel,Luc Ponc

Constitution

Frank H. T. Nelissen,Hans A. Heus,Sybren S. Wijmenga

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,Expression and Purification of RNA–Protein Complexes in ,,that allows efficient in vivo expression of recombinant RNA in .. We have extended the “tRNA scaffold” method to RNA/protein co-expression in order to express and purify RNA by affinity in native condition. As a proof-of-concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.

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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820191.jpg

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