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Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2021Latest edition Springer Science+Business Media, LLC, part of Spring

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發(fā)表于 2025-3-21 18:47:15 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱RNA Remodeling Proteins
副標(biāo)題Methods and Protocol
編輯Marc Boudvillain
視頻videohttp://file.papertrans.cn/821/820189/820189.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2021Latest edition Springer Science+Business Media, LLC, part of Spring
描述.This volume.?.provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components.?Written in the highly successful?.Methods in Molecular Biology.?series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls...?..Authoritative and cutting-edge, .RNA Remodeling Proteins: Methods and Protocols, Second Edition .aims to ensure successful results in the further study of this vital field..
出版日期Book 2021Latest edition
關(guān)鍵詞Chemical footprinting; Thermodynamic analysis; Helicases; eukaryotes; prokaryotes
版次2
doihttps://doi.org/10.1007/978-1-0716-0935-4
isbn_softcover978-1-0716-0937-8
isbn_ebook978-1-0716-0935-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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發(fā)表于 2025-3-21 21:37:04 | 只看該作者
1064-3745 ation advice from the experts.This volume.?.provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex
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Happy Birthday: 30 Years of RNA Helicases,y the structure of RNA molecules and/or the functionality of ribonucleoprotein complexes. Ultimately, the action of RNA helicases results in changes in gene expression that allow the cell to perform crucial functions. In this chapter, we review established and emerging concepts for DEAD-box and DExH
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Thermal Shift Assay for Characterizing the Stability of RNA Helicases and Their Interaction with Liof various ligands on that stability. It is particularly useful for the assaying of ligands that may stabilize oligomeric helicases, which rely on both substrates (oligonucleotides) and nucleotide cofactors (ATP analogues) for their stability in a functional state. In this chapter, we describe the r
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,High-Throughput Protein–Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhanchese interactions is crucial for delineating the mechanisms governing transcription, genome duplication, and translation. Here we describe a detailed protocol for the quantitative analysis of protein–nucleic acid interactions based on protein-induced fluorescence enhancement (PIFE). While PIFE has m
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Probing RNA Helicase Conformational Changes by Single-Molecule FRET Microscopy,nd total internal reflection fluorescence (TIRF) microscopy to follow the conformational cycling of DEAD-box helicases on the single molecule level, using the eukaryotic translation initiation factor eIF4A as an illustrative example. Confocal microscopy enables the study of donor-acceptor-labeled mo
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