Titlebook: RNA Nanostructures; Methods and Protocol Eckart Bindewald,Bruce A. Shapiro Book 2017 Springer Science+Business Media LLC 2017 RNA Architect

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Cotranscriptional Production of Chemically Modified RNA Nanoparticles,of siRNAs, RNA aptamers, and ribozymes. In vitro transcription of a mixture of dsDNA templates encoding all the subunits of the RNA nanoparticle may result in cotranscriptional self-assembly of the nanoparticle. Based on our experience with production of RNA nanorings, RNA nanocubes, and RNA three-w

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Design and Crystallography of Self-Assembling RNA Nanostructures,uctures. Designed base pair interactions allow complex nano-objects to self-assemble from simple RNA motifs. X-ray crystallography plays an important role in both the design and analysis of such RNA nanostructures. Here, we describe methods for the design and X-ray crystallographic structure analysi

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X-Aptamer Selection and Validation,lecular pathway targeting, biomarker discovery, or disease diagnosis by interacting with protein targets on the surface of cells or in solution. Such targeting is being used for imaging, diagnostic evaluation, interference of protein function, or delivery of therapeutic agents. Selection of aptamers

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,Synthesis of PLGA–Lipid Hybrid Nanoparticles for siRNA Delivery Using the Emulsion Method PLGA-PEG–meric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG–catio