Titlebook: RNA Mapping; Methods and Protocol M. Lucrecia Alvarez,Mahtab Nourbakhsh Book 2014 Springer Science+Business Media New York 2014 RNA boundar

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Identification of Actively Translated mRNAsomes), bind to a single mRNA in order to initiate translation and protein synthesis. In order to distinguish actively translated RNAs, total polysomes can be isolated from cell lysates and purified by centrifugation through sucrose density gradients. The polysome fraction represents all actively tra

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Mapping of Protein Binding RNA Elementson the separation of protein RNA mixtures through a native acrylamide gel. Compared to unbound RNA, RNA–protein complexes migrate slower through the gel resulting in a mass shift. The RNA EMSA can be used to identify unknown RNA–protein complexes, to map the RNA binding site of single proteins or de

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Single Nucleotide Mapping of RNA 5′ and 3′ Ends the length of protected fragment. Because of its high sensitivity and resolution, nuclease protection assay is the most effective procedure for mapping internal and external boundaries in mRNA compared to other RNA detection methods such as RT-PCR.

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